TRACERx-PHLEX: One-stop pipeline for robust multiplexed imaging analyses

TRACERx-PHLEX is a user-friendly pipeline for an end-to-end analysis of multiplex imaging data.

Citations

The pipeline is described in Magness et al. Nature Communications, 2024, doi: 10.1038/s41467-024-48870-5.

TRACERx-PHLEX is a modular pipeline for comprehensive analysis of the tumour microenvironment using multiplex imaging.

Check out the Quick start section for further information, including installation for how to get started with TRACERx-PHLEX.

TRACERx-PHLEX contains three submodules:

A module devoted to performing accurate nuclear and cellular segmentation and single cell measurement in multiplex images.

A module for cellular phenotyping from marker expression intensities derived from multiplex images.

A module for performing several types of automated spatial analysis.